This course contains information for researchers, animal technicians, and facility managers who are involved in setting up, maintaining, and optimizing breeding colonies of genetically-altered mice. Course participants should be familiar with the basics of mouse breeding and husbandry, as covered in Colony Management I: Breeding and Recordkeeping and Colony Management II: Daily Workflow.  After successfully completing this course, you should be able to:

1. Discuss how to start a colony.
2. Estimate the required size of a colony.
3. Estimate the total number of mice to be produced.
4. Describe how to obtain breeders.
5. Identify troubleshooting strategies.
6. Obtain embryos via superovulation.
7. Explain rescue strategies and assisted reproductive technologies.
8. Describe how to recovering live mice from frozen embryos or sperm.

AALAS has approved this course for 2.00 Continuing Education Units (CEUs) for completing this course and passing the examination.