Purpose:

This guideline describes best practices for mouse and rat anesthesia techniques. Dose ranges capture variation that may exist due to animal’s age, sex, strain, or individual responses. Dose requirements may vary depending on other drugs administered concurrently. This guideline is not intended to cover anesthetic overdose as a method for euthanasia.  Veterinary consultation is encouraged to determine the best anesthetic regimen prior to IACUC protocol submission.